上游充通大睾 SHANGHAI JIAO TONG UNIVERSITY 1896 1935 987 《现代材料科学实验方法》博士生课程（C050717) 多尺度关联的组织结构表征方法 (Correlative multi-scale characterization) 张澜庭 材料科学与工程学院 2017年5月2日 lantingzh@sjtu.edu.cn
材料科学与工程学院 School of Materials Science and Engineering 1896 1935 1987 2006 多尺度关联的组织结构表征方法 (Correlative multi-scale characterization) 张澜庭 材料科学与工程学院 2017年5月2日 lantingzh@sjtu.edu.cn 《现代材料科学实验方法》博士生课程（C050717）
图 Outline A brief introduction to analytical electron microscopy(courtesy Prof.Shipu Chen) Example of correlative multi-scale characterization(多尺度关联的组织结构表 征方法) http://cc.sjtu.edu.cn/G2S/site/frontiers of materials experimentation.html 材料科学与工程学院 3 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering Outline • A brief introduction to analytical electron microscopy (courtesy Prof. Shipu Chen) • Example of correlative multi-scale characterization (多尺度关联的组织结构表 征方法) 3 http://cc.sjtu.edu.cn/G2S/site/frontiers of materials experimentation.html
图 《现代材料科学实验方法》 OM PPMS XRD STM XRF AFM SEM sample Raman EPMA DSC TEM ICP XPS AES 材料科学与工程学院 4 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 《现代材料科学实验方法》 4 OM XRD XRF SEM EPMA TEM XPS AES ICP DSC Raman AFM STM PPMS sample
SEM TEM 500μm 材料科学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering SEM TEM 500 µm
盲人摸象困局 Matching scales SEM TEM 500μm 材料科学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering SEM TEM 500 µm 盲人摸象困局 Matching scales
多尺度相关显微分析的必要性 O TONG 以电沉积金属镍中的非正常晶粒长大为例： 1.晶粒尺寸(OM) {100} 2.非正常晶粒长大特征(SEM) 晶面 3.晶界特征(TEM) EBSD 光学显微镜 SEM/EBSD TEM/STEM/EDS √晶粒尺寸分布 √晶粒取向分布 √晶界/晶粒内成分分布 50μm :10μm 1μm Ni K Ni K Ni KI S Ko 特征定位 200 Sulfur rich phase School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 以电沉积金属镍中的非正常晶粒长大为例： 1. 晶粒尺寸（OM） 2. 非正常晶粒长大特征（SEM） 3. 晶界特征（TEM） 1 μm 光学显微镜 晶粒尺寸分布 50 μm 10 μm TEM/STEM/EDS 晶界/晶粒内成分分布 特征定位 SEM/EBSD 晶粒取向分布 Ni_50_500°C_1 h, matrix grain size 1.24µm large grain size 14.96 µm Ni_50_500°C_10 h, matrix grain size 2.38µm large grain size 181.88µm EBSD {100} 晶面 Sulfur-rich phase 多尺度相关显微分析的必要性
Correlative Light and Electron Microscopy (CLEM) In an integrated CLEM system,the sample is imaged using an electron beam and an optical light path simultaneously. Fluorescence microscope- highlight the regions of interest Electron microscope-obtain structural information at the nano- scale Carolien van Rijnsoever,Viola Oorschot Judith Klumperman,Nature Methods 5,973-980(2008) Pascal de Boer,Jacob P Hoogenboom Ben N G Giepmans,Nature Methsz,5(2015) School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering Correlative Light and Electron Microscopy (CLEM) • In an integrated CLEM system, the sample is imaged using an electron beam and an optical light path simultaneously. Carolien van Rijnsoever, Viola Oorschot & Judith Klumperman, Nature Methods 5, 973 - 980 (2008) Pascal de Boer, Jacob P Hoogenboom & Ben N G Giepmans, Nature Methods 12, 503–513 (2015) Electron microscope- obtain structural information at the nanoscale Fluorescence microscopehighlight the regions of interest
GFP-标记的NS5A(一 501 10m b 种亚基因) NS5A-GFP信号主要对 应于LDs和聚集在其附近 的DMV LD DMVs http://www.imaging-git.com/science/electron- and-ion-microscopy/correlative-light-and- 200nm electron-micro$ce砂学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering http://www.imaging-git.com/science/electronand-ion-microscopy/correlative-light-andelectron-microscopy GFP-标记的NS5A（一 种亚基因） NS5A-GFP信号主要对 应于LDs和聚集在其附近 的DMV
lo TON Part 2 CORRELATIVE MULTI-SCALE CHARACTERIZATION 材料科学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering CORRELATIVE MULTI-SCALE CHARACTERIZATION Part 2
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材料科学与工程学院 School of Materials Science and Engineering 12 225 kW 60 kW 动能回收电机：120 kW 热能回收电机：90 kW