配制Tris-甘氨酸SDs聚丙烯酰胺凝胶电泳分离胶溶液 溶液成分 总体积总体积总体积总体积总体积总体积 20ml 10.6ml 15.9ml 3%丙烯酰胺10mn1|20mn130m40m 6.0ml 1.5M Tris(pH 1.3ml 2.5ml 3.8ml 5.0n 6.3ml 7.5ml 10% SDS 0.05ml 0.1ml 0.2ml 0.25ml 0.3ml 10%过硫酸胺005 0.1ml015ml 0.25ml 0.3ml TEMED 0.004 0008ml0012ml0016ml 0.02ml 0.024ml 8% 2.3ml 4.6ml 6.9ml 11. 5ml 13.9ml 30%丙烯酰胺1.3ml 1.5M Tris(pH 1.3ml2.5ml38ml 5.0ml 6.3ml 7.5ml 10% SDS 0.1ml 0.25ml 0.3ml 10%过硫酸胺|005ml|0.ml TEMED 0.003ml0.006ml0.009ml 0.012ml 0.015ml 0. 018ml 11.9ml 30%丙烯酰胺1.7ml 3.3ml 8.3ml 100ml 1.5M Tris(pH 1.3ml 2.5ml38ml 5.0ml 6.3ml 7. 5ml 10% SDS 0.05ml 0.15ml 0.2ml 0.25ml 0.3ml 10%过硫酸胺005ml0ml0.15ml 0.25ml TEMED 0.002 0.004ml0.006ml0.008m 0. 01ml 0.012ml190 配制 Tris-甘氨酸 SDS 聚丙烯酰胺凝胶电泳分离胶溶液 溶液成分 总体积 5ml 总体积 10ml 总体积 15ml 总体积 20ml 总体积 25ml 总体积 30ml 6% 水 2.6ml 5.3ml 7.9ml 10.6ml 13.2ml 15.9ml 30%丙烯酰胺 1.0ml 2.0ml 3.0ml 4.0ml 5.0ml 6.0ml 1.5M Tris(pH 8.8) 1.3ml 2.5ml 3.8ml 5.0ml 6.3ml 7.5ml 10% SDS 0.05ml 0.1ml 0.15ml 0.2ml 0.25ml 0.3ml 10%过硫酸胺 0.05ml 0.1ml 0.15ml 0.2ml 0.25ml 0.3ml TEMED 0.004ml 0.008ml 0.012ml 0.016ml 0.02ml 0.024ml 8% 水 2.3ml 4.6ml 6.9ml 9.3ml 11.5ml 13.9ml 30%丙烯酰胺 1.3ml 2.7ml 4.0ml 5.3ml 6.7ml 8.0ml 1.5M Tris(pH 8.8) 1.3ml 2.5ml 3.8ml 5.0ml 6.3ml 7.5ml 10% SDS 0.05ml 0.1ml 0.15ml 0.2ml 0.25ml 0.3ml 10%过硫酸胺 0.05ml 0.1ml 0.15ml 0.2ml 0.25ml 0.3ml TEMED 0.003ml 0.006ml 0.009ml 0.012ml 0.015ml 0.018ml 10% 水 1.9ml 4.0ml 5.9ml 7.9ml 9.9ml 11.9ml 30%丙烯酰胺 1.7ml 3.3ml 5.0ml 6.7ml 8.3ml 10.0ml 1.5M Tris(pH 8.8) 1.3ml 2.5ml 3.8ml 5.0ml 6.3ml 7.5ml 10% SDS 0.05ml 0.1ml 0.15ml 0.2ml 0.25ml 0.3ml 10%过硫酸胺 0.05ml 0.1ml 0.15ml 0.2ml 0.25ml 0.3ml TEMED 0.002ml 0.004ml 0.006ml 0.008ml 0.01ml 0.012ml