重组DNA导入大肠杆菌的方法 平板培养基培养细菌 10-100μL转化漩 z Suspension of 涂于合抗菌素的平板 Suspension spread on bacterial cells petri plate with agar gel Incubate from 1 to 2 days 37℃过夜 Petri plat with agar gel Single cells Visible colonies (not visible to naked eye) (each a clone of the corresponding single cell)一、重组DNA导入大肠杆菌的方法 平板培养基培养细菌 10-100L转化液 涂于含抗菌素的平板 37℃过夜