B Specific functions localized within adm af low oemddaty the mit by disruption of the organelle the content of the wmermemdea and fractionation Figure 14-6 Fractionation of purified on leave the cinerea mitochondria into separate ing action components. These techniques have made it possible to study the different proteins in each mitochondrial compartment. The method shown traneler to a mosan od which allows the processing of large numbers of mitochondria at the same time takes advantage tasty gaunt oewtrtuoaton of the fact that in media of low osmotic strength tom na cente matre an ita runeng ambra water flows into mitochondria and greatly expands the matrix space (yellow). While the cristae of the inner membrane allow it to unfold disruption and cermtnfugata tonale wnnt to accommodate the expansion, the outer membranewhich has no folds to begin withbreaks, releasing a structure composed of only the inner membrane and the matrixFigure 14-6 Fractionation of purified mitochondria into separate components. These techniques have made it possible to study the different proteins in each mitochondrial compartment. The method shown, which allows the processing of large numbers of mitochondria at the same time, takes advantage of the fact that in media of low osmotic strength water flows into mitochondria and greatly expands the matrix space (yellow). While the cristae of the inner membrane allow it to unfold to accommodate the expansion, the outer membranewhich has no folds to begin withbreaks, releasing a structure composed of only the inner membrane and the matrix. B. Specific functions localized within the Mit by disruption of the organelle and fractionation