Question 3 continued You learn that cell line l is a skin cancer cell line. The region you identified as deleted on chromosome 4 in these cells normally contains a gene called p16 d)What is the role of the p16 gene product in the normal cell based on the information above? You obtain another cell line(cell line 3)that has one wild-type copy of chromosome 4 and one mutant copy of chromosome 4(as described above in cell line 1) e)Will cell line 3 display a cancerous phenotype when grown in the presence of growth factors? Yes/No(Circle one Explain briefly f)Will cell line 3 display a cancerous phenotype when grown in the absence of growth factors? Yes/No(Circle one. Explain briefly Part Ill g)Cell line 2 is a breast cancer cell line that expresses a mutant version of a receptor protein called KIT. Choose from the following options to explain the role of KIT in normal cells Circle one Activation of kit causes cells to die Activation of Kit promotes progression through the cell cycle Activation of kit has no effect on the cell cycle Activation of kit causes cells to enter go h) Specifically how could a point mutation in the gene encoding the Kit receptor cause the abnormal behavior depicted in Part I6 Question 3 continued You learn that cell line 1 is a skin cancer cell line. The region you identified as deleted on chromosome 4 in these cells normally contains a gene called p16. d) What is the role of the p16 gene product in the normal cell based on the information above? You obtain another cell line (cell line 3) that has one wild-type copy of chromosome 4 and one mutant copy of chromosome 4 (as described above in cell line 1). e) Will cell line 3 display a cancerous phenotype when grown in the presence of growth factors? Yes/No (Circle one.) Explain briefly. f) Will cell line 3 display a cancerous phenotype when grown in the absence of growth factors? Yes/No (Circle one.) Explain briefly. Part III g) Cell line 2 is a breast cancer cell line that expresses a mutant version of a receptor protein called KIT. Choose from the following options to explain the role of KIT in normal cells. Circle one. Activation of KIT causes cells to die Activation of KIT promotes progression through the cell cycle. Activation of KIT has no effect on the cell cycle. Activation of KIT causes cells to enter G0. h) Specifically how could a point mutation in the gene encoding the KIT receptor cause the abnormal behavior depicted in Part I