Question 4 you know in yeast that mitosis takes one hour you decide to further study the cell cycle in yeast cells using radioactive dTTP. Cells grown in radioactive dTTP incorporate this radioactive nucleotide into their DNA. you label a population of asynchronously growing yeast cells by adding radioactive dTTP to the medium for one minute. you then replace this medium with medium containing unlabelled dTTP and continue growing the cells. At one hour time points following the replacement you count the number of radioactively labeled cells in mitosis. your data is shown below 12000 0000 4000 234567891011121314151617181920212223 Time(in hours) after addition of unlabelled medium a)Cells are in which phase of the cell cycle when incorporating radioactive dTTP into their DNA?(Circle one. Go phase M phase Lunar phase b)Estimate the length of the G2 phase from the graph.(Circle one. O hrs 2-3 hrs 96-7 hrs 9-10 ~13-14hrs 20 hrs w22 hrs c)Estimate the length of the s phase from the graph.( Circle one. Can't o hrs N2-3 hrs -6-7 hrs 2 hrs d) Estimate the duration of the cell cycle. (Circle one Can't be O hrs w2-3 hrs *6-7 hrs ~9-10 11-12hrs ~13-14hrs c22 hrs e)Estimate the length of the G1 phase from the graph. ( Circle one. O hrs 2-3 hrs M6-7 hrs ~9-10 ~11-12hrs ~13-14hrs w22 hrs7 Question 4 You know in yeast that mitosis takes one hour. You decide to further study the cell cycle in yeast cells using radioactive dTTP. Cells grown in radioactive dTTP incorporate this radioactive nucleotide into their DNA. You label a population of asynchronously growing yeast cells by adding radioactive dTTP to the medium for one minute. You then replace this medium with medium containing unlabelled dTTP and continue growing the cells. At one hour time points following the replacement you count the number of radioactively labeled cells in mitosis. Your data is shown below. 0 2000 4000 6000 8000 10000 12000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Time (in hours) after addition of unlabelled medium Number of labelled cells in mitosis a) Cells are in which phase of the cell cycle when incorporating radioactive dTTP into their DNA? (Circle one.) G0 phase G1 phase G2 phase M phase S phase Lunar phase b) Estimate the length of the G2 phase from the graph. (Circle one.) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs c) Estimate the length of the S phase from the graph. (Circle one.) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs d) Estimate the duration of the cell cycle. (Circle one) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs e) Estimate the length of the G1 phase from the graph. (Circle one.) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs