E+ESE+P V。=k2[ES] Rate of formation of ES=kES] (15) Rate of breakdown of ES =(k_1+k2)ES (16) George Briggs and John Haldane suggested the steadystate assumption in 1924. 稳态假说：酶一底物复合物形成及分解的速度相同 k1E S=(k-1+k2)ESGeorge Briggs and John Haldane suggested the steadystate assumption in 1924. 稳态假说：酶－底物复合物形成及分解的速度相同 Vo=k2［ES］