Example peptides used to modulate cell adhesion on biomaterials Peptide Derived from Conjugate receptor Role References Laminin a-chain LBP110(110 KDa Cell-ECM J Biol Chem. 264, 16174 (1989) RGD Laminin a-chain Multiple integrins Cell-ECM J Cell Physiol. 146, 451(1991) YIGSR amini B1-chain a1B1 and a3Bl Cell-ECM Cell 48, 989(1987): Arch ntegrins adhesion Biochem Biophys. 272, 39 (1989);J. Biol Chem.268,8053 RNIAELKDI amanin y-chain unknown Cell-ECM FEBS Lett. 244, 141(1989) HAV N-cadherin N-cadherin Cell-cell Dev BioL. 139, 227(1990) DGEA Type I collagen a2B, integrin Cell-ECM VAPG Elastase Elastase recept tor adhesion KQAGDV Fibrinogen y-chain B3 integrins Cell-ECM adhesion Applying the'comb polymer approach on biodegradable materials n Hydrolytically-degradable polymer TISSUE ENGINEERING scaffold This general approach has also been applied to fully biodegradable polymers use peptide or side chain-bearing ring monomers to create biodegradable backbones with PEG side chains that can be functionalized (not trivial Lecture 3- Biological Recognition 14 of 15Example peptides used to modulate cell adhesion on biomaterials Peptide sequence Derived from Conjugate receptor Role References IKVAV Laminin α-chain LBP110 (110 KDa laminin binding protein) Cell-ECM adhesion J. Biol. Chem. 264, 16174 (1989) RGD Laminin α-chain, fibronectin, collagen Multiple integrins Cell-ECM adhesion J. Cell Physiol. 146, 451 (1991) YIGSR Laminin β1-chain α1β1 and α3β1 integrins Cell-ECM adhesion Cell 48,989 (1987); Arch. Biochem. Biophys. 272, 39 (1989); J. Biol. Chem. 268, 8053 (1993) RNIAEIIKDI Laminin γ-chain unknown CM adhesion FEBS Lett. 244, 141 (1989) HAV N-cadherin herin adhesion Dev. Biol. 139, 227(1990) DGEA Type I collagen α2β1 integrin Cell-ECM adhesion VAPG tase Elastase receptor Cell-ECM adhesion KQAGDV Fibrinogen γ-chain β3 integrins Cell-ECM adhesion Cell-E N-cad Cell-cell Elas ƒ Applying the ‘comb polymer’ approach on biodegradable materials: QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. Comb polymer thin film coating Hydrolytically-degradable polymer QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. TISSUE ENGINEERING scaffold o This general approach has also been applied to fully biodegradable polymers ƒ use peptide or side chain-bearing ring monomers to create biodegradable backbones with PEG side chains that can be functionalized (not trivial) Lecture 3 – Biological Recognition 14 of 15