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WOvD世华人化杀志 lideng. com/wcjd/ch/index.aspx 世界华人消化杂志2016年7月18日;24(20):3142-314 com/esps/helpdesk. asp ISSN 1009-3079(print) IsSN 2219-2859(online DO:10.1569/wdv24i20.3142 o 2016 Baishideng Publishing Group Inc. All rights reserved. 肠道菌群的检测方法及研究进展 刘玉婷,郝微微,温红珠,邵兰君 ■背景资料 刘玉婷温红珠,邵兰君,上海中医药大学附属龙华医院消Road,Shanghai200437,China.hao2364ahotmail.com 肠道菌群对人体化科上海市200032 的健康起着保护 Received: 2016-04-25 作用,但是菌群郝微微,上海中医药大学附属岳阳中西医结合医院消化科 Revised:2016-06-2 在数量、种属、上海市200437 Accepted:2016-06-27 比例等方面失调 Published online: 2016-07-18 与疾病的发生有刘玉婷硕土研究生,主要从事中医约防治青肠疾病的研究 关,随着检测技 术的进展,对肠 道菌群的认识也 床研究基地龙医团队基金资助项目 TD_: Abstract 在不断推进,本 There are a large number of microorganisms 分子生物学技术米作若贡献分布;在文中号元三,理集分中 the human intestine, and they play an 玉婷完成;资料筛选由温红珠与召兰君完成;综述由刘玉婷 important role in digestion and absorption, 检测方法作简要完成 energy metabolism, immune regulation 乍者:郝微微,主任医师,200437,上海市甘河路110号 disease-resistant ability and so on, and are 医药大学附属岳阳中西医结合医院消化科 associated with the development of many diseases. The intestinal flora is of important significance for clinical treatment. This review 修回日期:2016-06 focuses on a variety of molecular biology 接受日期:2016-06-27 technologies for intestinal flora research, such 在线出版日期:2016-07-18 as polymerase chain reaction(PCR), PCR based 16 S rDNA fingerprinting, fluorescence Intestinal flora detection n situ hybridization, gene chip, metagenome methods and research sequencing and so on, as well as the prospects for the research of intestinal flora advances The Author(s) 2016. Published by Baishideng Yu-ling Lu, We-We Hao, Hong-Zhu Wen, Lan-Jun Shao Publishing Group Inc. All rights reserved Wen, Lan-Jun Shao, Department Key Words: Intestinal flora; Detection methods; TCM. Shanghai 200032. China Liu YT, Hao ww, Wen Hz, Shao L Affiliated to Shanghai University of TCM, Detection methods and research Huaren Xiaohua Zazhi 2016; 24 (20) URL http://www.wignet.com/1009-3079/full/v24/ Supportedby:NationalNaturalScienceFoundationofi20/3142.htmDol:http://dx.doi.org/10.11569/wcjd China. No 62: Nation Clinical Research v.1203142 Base Longyi Team Project, No. LYTD-09, 2012 Shanghai Natural Science Foundation, No. 12ZR1432000 同行评议者 Correspondence to: Wei-Wei Hao, Chief Physician, j54 谭周进,教授,湖 Department of Gastroenterology, Yueyang Hospital 南中医药大学 Affiliated to Shanghai University of TCM, 110 Ganhe人的肠道中栖息着大量微生物,在消化吸 3142 2016-07-18 Volume 24 I Issue 20 IWCJD|www.wjgnet.com 3142 2016-07-18|Volume 24|Issue 20| ■背景资料 肠道菌群对人体 的健康起着保护 作用, 但是菌群 在数量、种属、 比例等方面失调 与疾病的发生有 关, 随着检测技 术的进展, 对肠 道菌群的认识也 在不断推进, 本 文就几种常用的 分子生物学技术 检测方法作简要 阐述. 刘玉婷, 温红珠, 邵兰君, 上海中医药大学附属龙华医院消 化科 上海市 200032 郝微微, 上海中医药大学附属岳阳中西医结合医院消化科 上海市 200437 刘玉婷, 硕士研究生, 主要从事中医药防治胃肠疾病的研究. 基金项目: 国家自然科学基金资助项目, No. 81403362; 国 家中医临床研究基地龙医团队基金资助项目, No. LYTD-09; 2012年度上海市自然基金资助项目, No. 12ZR1432000. 作者贡献分布: 本文由郝微微指导完成; 资料搜集与分析由刘 玉婷完成; 资料筛选由温红珠与邵兰君完成; 综述由刘玉婷 完成. 通讯作者: 郝微微, 主任医师, 200437, 上海市甘河路110号, 上海中医药大学附属岳阳中西医结合医院消化科. hao2364@hotmail.com 收稿日期: 2016-04-25 修回日期: 2016-06-21 接受日期: 2016-06-27 在线出版日期: 2016-07-18 Intestinal flora: Detection methods and research advances Yu-Ting Liu, Wei-Wei Hao, Hong-Zhu Wen, Lan-Jun Shao Yu-Ting Liu, Hong-Zhu Wen, Lan-Jun Shao, Department of Gastroenterology, Longhua Hospital Affiliated to Shanghai University of TCM, Shanghai 200032, China Wei-Wei Hao, Department of Gastroenterology, Yueyang Hospital Affiliated to Shanghai University of TCM, Shanghai 200437, China Supported by: National Natural Science Foundation of China, No. 81403362; National TCM Clinical Research Base Longyi Team Project, No. LYTD-09; 2012 Shanghai Natural Science Foundation, No. 12ZR1432000. Correspondence to: Wei-Wei Hao, Chief Physician, Department of Gastroenterology, Yueyang Hospital Affiliated to Shanghai University of TCM, 110 Ganhe Road, Shanghai 200437, China. hao2364@hotmail.com Received: 2016-04-25 Revised: 2016-06-21 Accepted: 2016-06-27 Published online: 2016-07-18 Abstract There are a large number of microorganisms in the human intestine, and they play an important role in digestion and absorption, energy metabolism, immune regulation, disease-resistant ability and so on, and are associated with the development of many diseases. The intestinal flora is of important significance for clinical treatment. This review focuses on a variety of molecular biology technologies for intestinal flora research, such as polymerase chain reaction (PCR), PCR￾based 16 S rDNA fingerprinting, fluorescence in situ hybridization, gene chip, metagenome sequencing and so on, as well as the prospects for the research of intestinal flora. © The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. Key Words: Intestinal flora; Detection methods; Molecular biology techniques Liu YT, Hao WW, Wen HZ, Shao LJ. Intestinal flora: Detection methods and research advances. Shijie Huaren Xiaohua Zazhi 2016; 24(20): 3142-3148 URL: http://www.wjgnet.com/1009-3079/full/v24/ i20/3142.htm DOI: http://dx.doi.org/10.11569/wcjd. v24.i20.3142 摘要 人的肠道中栖息着大量微生物, 在消化吸 肠道菌群的检测方法及研究进展 刘玉婷, 郝微微, 温红珠, 邵兰君 在线投稿: http://www.baishideng.com/wcjd/ch/index.aspx 帮助平台: http://www.wjgnet.com/esps/helpdesk.aspx DOI: 10.11569/wcjd.v24.i20.3142 世界华人消化杂志 2016年7月18日; 24(20): 3142-3148 ISSN 1009-3079 (print) ISSN 2219-2859 (online) © 2016 Baishideng Publishing Group Inc. All rights reserved. 文献综述 REVIEW ® ■同行评议者 谭周进, 教授, 湖 南中医药大学
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