N-methylcarbamate insecticide residues, including carbamate metabolites, are extracted with methanol. The extract is cleaned up by partitioning and column chromatography on a charcoal/Celite column. Residues are selectively determined
Pesticide multiresidue methods (MRMs) are capable of simultaneously determining more than one residue in a single analysis; this multiresidue capability is provided by a GLC or HPLC determinative step that separates residues from one another before detection. The MRM concept is raised to a higher dimension when a single extract is examined with more than one chromatographic determinative
The expressed purpose of the Pesticide Analytical Manual is to publish analytical methodology used by the FDA in enforcing pesticide tolerances. To understand FDA’s application of methodology published in the manual, it is important to
Recovery of Chemicals Through Method 401 (E1-E2 + C1 + DL1) (methanol extraction, cleanup with partitioning and charcoal/Celite column, HPLC with post-column derivatization and fluorescence detection)
Caution: Use this table only as a quick reference for tentative identification of residues found in samples analyzed by the most commonly used PAM I multiresidue methods. Always compare the residue to a standard chromatographed in your own gas chromatograph
Transmittal No. 96-E1 (9/96) Form FDA 2905a (6/92) INDEX: METHODS–1 Pesticide Analytical Manual Vol. I INDEX: METHODS Index to PAM I Methods, by Chemicals Tested for Recovery Codes: C: complete (recovery); P: partial; S: small; V: variable; R: recovered; NR: not recovered
Multiresidue methodology by definition requires determinative steps capable of separating analytes from one another so each can be detected and measured individually. Both gas-liquid chromatography (GLC) and high performance liquid
