PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY ELSEVIER Progress in Nuclear Magnetic Resonance Spectroscopy 38(2001)83-114 www.elsevier.nl/locate/pnmrs NMR studies of protein -DNA interactions N. Jamin“,F.Ton
Many intracellular proteins are required to interact or bind to other macromolecules within a bind DNA. Such proteins often have conserved structural features, called motifs, whis at can cell in order to function properly. One class of such proteins is comprised of proteins tha interact with DNA. One such motif consists of two closely aligned a-helices(shown as cylinders below)that each have leucine-rich regions. This motif is referred to as a leucine
◼ All DNA is recombinant DNA. ◼ Genetic exchange works constantly to blend and rearrange chromosomes, most obviously during meiosis, when homologous chromosomes pair prior to the first nuclear division. ◼ During this pairing, genetic exchange between the chromosomes occurs. This exchange, classically termed crossing over, is one of the results of homologous recombination
Transcription is very similar to DNA replication but there are some important differencesi 1 RNA is made of ribonucleotides 2. RNA polymerase catalyzes the reaction 3. The synthesized RNa does not remain base-paired to the template DNA strand 4. Less accurate(error rate: 10-4)
Q1. Prokaryotic cell E. coli consists of a single closed-circular DNA molecule of 4.6 x 106 bp long. This DNA molecule would be 1.6 mm if the double helix is relaxed. Please describe what strategies does E. coli use to pack such a long molecules into its nucleoid less than a micrometer (mm) in diameter. (20 points)
Q1. Prokaryotic cell E. coli consists of a single closed-circular DNA molecule of 4.6 x 106 bp long. This DNA molecule would be 1.6 mm if the double helix is relaxed. Please describe what strategies does E. coli use to pack such a long molecules into its nucleoid less than a micrometer (mm) in diameter. (20 points)
Molecular biologists routinely use restriction enzymes as key reagents for a variety of applications including genomic mapping, restriction fragment length polymorphism (RFLP) analysis, DNA sequencing, and a host of recombinant DNA methodologies. Few