NMR supplement The second decade into the third millenium Kurt Wuthrich NMR spectroscopy is one of the principal experimental techniques of structural biology, with abilities to determine atomic resolution structures as well as investigate dynamics and intermolecular interactions of

MACINETIC RESONANCE ELSEVIER Progress in Nuclear Magnetic Resonance Spectroscopy 32(1998)287-387 The use of nmr methods for conformational studies of nucleic acids Sybren S. Wijmenga*, Bernd N M. van Buuren Umed University, Department of Medical Biochemistry and Biophysics, S 90187 Umed, Sweden

NMR spectroscopy in structure-based drug design Gordon CK Roberts NMR methods for the study of motion in proteins continue Structure and dynamics of the binding site improve, and a n d complexes Methodological developments in NMR of macromolecules relevant to drug design

NMR of drugs and ligands bound to receptors Anthony Watts NMR methods are now able to give detailed structural, dynamic of these still not identified or classed into families [4\]. and electronic information about drugs and ligands while

TSINGHUA SCIENCE AND TECHNOLOGY ssN100702142325pp43-445 Volume 5, Number 4, December 2000 Interaction of Divalent Metal lons with the Adenosine Triphosphate Measured using nuclear magnetic resonance

NMR supplement Hendrickson, W-A& Wuthrich, K Macromolecular 16. Leahy, D.J., Aukhil, I.& Erickson, H.P. 2.0 Cell 84 A K.D. thanks the Wellcome Trust and The queens 2D0i9 Riddiough. G. Nature Struct. Biol 18. sakaning. AK Kean. cel l8r.597-605i(eRw& by the Wellcome Trust. The authors acknowledge

6 The noe effect c Gerd Gemmecker, 1999 Nuclear OVERHAUSER Effect(NOE): resonance line intensity changes caused by dipolare cross- relaxation from neighbouring spins with perturbed energy level populations To understand the nature of the NOE, we have to look at a two-spin system I' and

4 DQF-COSY, Relayed-COSY, TOCSY Gerd Gemmecker 1999 Double-quantum filtered cOSY The phase problem of normal COsY can be circumvented by the dQF-COSY, using the MQc term generated by the second 90 pulse Ilz cos( @2t1) cos( t1) polarization

2 Basis Principles of FT NMR C Gerd Gemmecker 1999 Nuclei in magnetic fields Atomic nuclei are composed of nucleons, i.e., protons and neutrons. Each of these particles shows a property named\spin\(behaving like an angular momentum) that adds up to the total spin of the

蛋白的膜拓扑学研究 蛋白质的晶体学研究: X-ray; EM 蛋白质的单颗粒研究: EM; AFM 蛋白质结构的波谱技术:荧光; CD; NMR