Chapter 5 Macromolecules and Molecular Genetics DNA Replication Complementary base pairing Semiconservative Template and primer From 5'-phosphate to the 3'-hydroxyl end DNA polymerase and ligase A short stretch of RNA serves as primer Primase: a specific RNA-polymerizing enzyme
Peptidoglycan Synthesis Transport of peptidoglycan precursors across the cytoplasmic. membrane to the growing point of the cell wall Outside Peptidoglycan Growing point of cell wall
Chapter 4 Metabolism, Biosynthesis and Nutrition Microbial Nutrition Laboratory Culture of Microorganisms Sterilization. Aseptic Technique, and Pure Cultures
Genetic Engineering and Biotechnology Genetic engineering: Isolation manipulation and expression of genetic materials. Gene cloning: Isolation and purification of specific genes In vitro recombination=recombinant DNA
1. Rodney F. Boyer, Modern Experimental Biochemistry, Addison-Wesley Publishing Company, Inc., 2000. 2. Keith Wilson and John M. Walker, Principles and techniques of practical biochemistry, Fourth edition, Cambridge University Press, 1994
Chapter 7 Regulation of Gene Expression Regulation of Enzyme Activity Constitutive enzymes -Enzymes induced by specific circumstances Two major modes of regulation in the cell Control the activity of preexisting enzymes Control the amount of an enzyme
