X-Ray diffraction is an important and powerful tool for determining the solid state structure of compounds. Modern advances have made data collection and structure solution almost routine for many small molecules To use this technique, however, good quality single crystals are still needed. In this exercise you will experiment with the art of growing single crystals

dscnibed in these materials are potentially hazardous and require a high level ofsafcty training special facilitics and equ by approp lividuak You bear the sole responsibility, liability, and risk for the in plementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of amy of the matcrial presented. Legal Notices

For a two-solvent recrystallization, you should have one solvent (solvent #1) in which your desired compound is soluble at the boiling point. The second solvent(solvent #)should induce crystallization when added to a saturated solution of your compound in the primary solvent. Reference:

Thin Layer Chromatography (TlC) is an extremely useful technique for monitorin reactions. It is also used to determine the proper solvent system for performing separations using column chromatography. tLC uses a stationary phase, usually alumina or silica, that is highly polar(standard) or non-polar(reverse phase). The mobile phase is a solvent whose polarity you will choose. In 5.301, and in most lab

Working on a vacuum manifold: See LLP chapter 9.2 for an excellent discussion on working with air-sensitive reagents, including how to use a two-way manifold. De-gassing solvents: The best way to remove water and oxygen from a solvent is to distill it over an appropriate drying agent (such as sodium). This can sometimes be a lengthy (and dangerous)task. Since we

Overview: As you may discover, growing single crystals takes patience as well as an artful hand Crystallizations can be very sensitive to temperature and minor disturbances. Therefore, you will be encouraged to try several different temperatures using otherwise identical conditions, and to always find a quiet undisturbed location to promote crystal growth. Here are some tried and true tips to get

WARNING NOTICE: The erperimants descnbed in these materials are potentially hao ardous and require a high level ofsafcty training special facilitics and equipment, and supervision by approprinte individuals. You bear the solc responsibility, liability, and risak for the implemcntation of such safdty procedunes and measures. MrT shall have no responsiblity, liability, risk for the content or implemcutation of amy of the matcrial presented. Legal Notices

大肠杆菌常规培养 大肠杆菌在LB( Luria-Bertani-)液体培养基中,37℃水浴摇床培养12-16小时在LB固 体培养基中,置于37℃培养箱培养12-16小时。如果培养用于提取具有氨苄青霉素(Amp)抗性 基因质粒(如pUC18)的菌体,则需在培养基中加入100mg/ml的氨青霉素

说明: 仪器分析是我校化学专业开设的一门应用性的专业基础课程。开设的目的是使学 生在学习分析化学的基础上,学习和掌握仪器分析的基本原理、基本方法、特点和应 用范围,了解现代分析仪器的发展方向。加强对其它课程所学有关知识和实验操作技 能的理解和应用。开阔学生的知识面。提高学生适应各种工作的能力,为今后的工作 打好基础

遗传学是研究生物遗传和变异规律、探索生命奥秘的理论科学,是高等农业院校所设的 一门重要的专业基础课,是植物、动物和微生物育种及相关课程的理论基础。 《普通遗传学》是农学、园艺、园林专业的必修专业基础课。通过本课程学习,要求学 生掌握遗传学的基本原理,掌握对动、植物和微生物进行遗传分析的一般方法,掌握基本的 实验操作技术,为进一步学习有关专业课程和遗传学的分支学科奠定较好的遗传学基础知识