I Systems Microbiology(13 Lectures 7hece∥ as a we∥}- stirred boc/ nem/ca/ reactor Introduction 2 Chemical kinetics, Equilibrium binding, cooperativity L3 Lambda phage L4 Stability analysis L5-6 Genetic switches

Note final ps For question 2(a)the value of k'in the final equation(Eq 3b) needs to be rescaled Tuesday lecture will be for questions on the last problem set Juan)

Review L15 Turing-Gierer-Meinhardt models Local excitation, global inhibition

creation of membrane associated minD first order first order autocatalytic inhibited by (linear, oligomerization) membrane minE, MM)

Organizational Remarks: ps#2,1b: Correction: Plot k, and for L= 0...2/K L (NOT: Plot ka and for L 0...2KL) Tomorrow's recitation topic: 'PS #2 support

Fine tuned model for perfect adaptation spiro et a1.PNAs94,7263-7268(1997) A model of excitation and adaptation in bacterial chemotaxis

L4: Cooperativity introduction 2 phage model n phage model (Hasty et al.) as example for applying mass action law

麻省理工大学：《Systems Biology》Review lecture 2 Michaelis-Menten kinetics

Typical Length Scales in Biology IA lO microtubule DNA width width typical animal cell length of DNA in a chromosome

Microbial molecular biology and genetics A clone: a population of cells that are derived asexually from a parental cell and are genetically identical. Genome: all the genes present in cell or virus. Central dogma:dna→rna→ protein transcription translation