Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer
Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers
Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions
Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures
In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??
