Announcements Power point lecture notes available on bcmp200 Website For further reading Nucleic Acids: Structures, Properties, and Functions vA Bloomfield D.M. Crothers Ⅰ. Tinoco
For further reading: Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D.M. Crothers I. Tinoco Announcements: Power Point Lecture Notes Available on BCMP200 Website
Interference Footprinting DNA+MMS● Cleave backbone a modified bases saturating Protein] ) ★ Gel shift
Interference Footprinting DNA + MMS + Saturating [Protein] Gel Shift 14 Cleave backbone @ modified bases
Chromatin is the last refuge of scoundrels
“Chromatin is the last refuge of scoundrels
A Mitotic human chromosome DNA length:4.6x 10 bp=1.5 cm Chromosome length: 2 um Compaction ratio=8000
A Mitotic Human Chromosome DNA length: 4.6 x 107 bp = 1.5 cm Chromosome length: 2 µm Compaction ratio = 8000 1
Chromatin DNA DNA Compaction Chromatin Accessibility Remodeling Machines NIH
Chromatin DNA Compaction DNA Chromatin Accessibility Remodeling Machines NIH
Basic Unit of Chromatin Structure: Polynucleosome Compaction ratio=6 2
Basic Unit of Chromatin Structure: Polynucleosome Compaction ratio = 6 2
Limited Micrococcal Nulcease Digestion of Chromatin Generates a 200 bp ladder 605→ 405→ 205→ 3
Limited Micrococcal Nulcease Digestion of Chromatin Generates a ~200 bp Ladder 3
Operational Definititions of Nucleosome Particles Linker DNA(variable) H HI H 200bp 165bp 146bp HI HI Chromatin Mononucleosome Chromatosome Core Particle Micrococcal Nuclease Digestion Time
H1 H1 H1 H1 H1 H1 Micrococcal Nuclease Digestion Time Mononucleosome Chromatosome Core Particle Linker DNA (variable) Operational Definititions of Nucleosome Particles Chromatin 4
The 30 nm Chromatin fiber Compaction ratio =50
The 30 nm Chromatin Fiber Compaction ratio = 50 5
A Mitotic human chromosome DNA length:4.6x 10 bp=1.5 cm Chromosome length: 2 um 30 nm filament Compaction ratio=8000
A Mitotic Human Chromosome DNA length: 4.6 x 107 bp = 1.5 cm Chromosome length: 2 µm Compaction ratio = 8000 1 30 nm filament
