There are many ways of cloning a disease gene, depending on what is known about the disease. We will show several typical examples in this part of the lecture
Using mutants to analyze the pathway or functions of genes Mutants have to be generated to analyze the pathway or functions of genes Saturation screening Overexpression Suppressor mutations Synthetic lethals Nonallelic noncomplementation
F and partial diploids The F-factor in a Hfr chromosome can excise out to become an autonomously replicating plasmid If a segment of the chromosome becomes part of the F pp lasmid during excision, the new element is called an F plasmid
