20.1 Introduction 20.2 Eukaryotic RNA polymerases consist of many subunits 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a universal factor 20.8 TBP binds DNA in an unusual way

8.10 Signal transduction and two-component regulatory system VI. Differences between prokaryotes and eukaryotes in gene expression V. RNA-Based Regulation 8.11 RNA Regulation and Antisense RNA 8.12 Riboswitches 8.13 Attenuation 弱化作用

一.概论 1.转录的反应 定义:从DNA区段基因中的一条链为模板,在RNA聚合酶的催化下,以四种核苷酸为原料,按照A-U,-C配对原则合成RNA

第一节 核酸的化学组成 一、戊糖 二、碱基 三、核苷 四、核苷酸 五、多磷酸核苷酸 六、环化腺苷酸 第二节 DNA分子的结构 一、DNA分子大小 二、DNA的碱基组成 三、DNA的一级结构 四、DNA的二级结构 五、DNA的三级结构 六、DNA的性质 第三节 RNA分子的结构 一、RNA的类型 二、RNA的碱基组成 三、RNA的结构

Transcription is very similar to DNA replication but there are some important differencesi 1 RNA is made of ribonucleotides 2. RNA polymerase catalyzes the reaction 3. The synthesized RNa does not remain base-paired to the template DNA strand 4. Less accurate(error rate: 10-4)

复旦大学：《基因组学》课程教学资源（课件讲稿）第08章 RNA的修饰与加工（小分子RNA——small RNA microRNA）

23.1 Introduction 23.2 Group I introns undertake self-splicing by transesterification 23.3 Group I introns form a characteristic secondary structure 23.4 Ribozymes have various catalytic activities 23.5 Some introns code for proteins that sponsor mobility 23.6 The catalytic activity of RNAase P is due to RNA 23.7 Viroids have catalytic activity 23.8 RNA editing occurs at individual bases

● 基本概念 ● 转录起始： RNA聚合酶、启动子 ● 转录的基本过程 ● 转录后加工 ● 原核生物与真核生物mRNA的特征比较 ● RNA合成与DNA合成异同点

一、三种不同的RNA聚合酶 二、RNA 聚合酶的组成 三、真核RNA聚合酶的活性

第一章 绪论 Chapter 1 Introduction 第二章 基因和染色体 Chapter 2 DNA and Chromosomes 第三章 生物信息的传递(上)——From DNA to RNA Chapter 3 Transcription — From DNA to RNA 第四章 生物信息的传递(下)——From mRNA to Protein Chapter 4 Translation: From RNA to Protein