要熟悉流管柱层析法需要适当的练习。此影片将会让从你填充 的适当条件技巧开始、填充及进行管柱层析,甚至用管子做一个微 管柱层析。 It takes considerable practice to master the art of \running a column\. This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet

发现一些薄层分析法更复杂的技巧---剪玻璃TLC板,用火 焰造TLC染色剂点棍,使用TLC染色剂以使点显像。 Discover a few of the more complicatec skills related to thin layer chromatography cutting glass TLC plates, flame-pulling spotters, and using TLC stains to visualize spots

This handout describes standard extraction and washing protocols that can be applied to virtually any crude reaction mixture. Aqueous washings are done to remove water soluble impurities from organic products since normally the compound that you desire will be dissolved in Reference

Flash column chromatography is a quick and (usually) easy way to separate complex mixtures of compounds. We will be performing relatively large scale separations in 5.301,around 1.0 g of compound. Columns are often smaller in scale than this and some of you will experience these once you move into the research lab. Column chromatography uses the same principles

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将液体从固体中分开来的最简易方法是什么?过滤在此影片中 将学习如何有效的用重力过露及真空过滤来进行。 The easiest way to separate a liquid from a solid? Filtration! Learn how to effectively carry out gravity and vacuum filtrations in this video

在本影片中学习如何熟悉滴定技术---用酚?在酸/碱滴定中的详 细示范。 Learn how to master the art of titration in this video-with a detailed demonstration of an acid/base titration using phenolphthalein

再结晶须有耐心,但它是值得的!此影片让你用一种溶剂与两种 溶剂的再结晶方法从溶解度测试到冷过滤来经验此过程 Recrystallization takes patience, but it's worth it! This video walks you through the procedure, from solubility tests to cold filtration, with information on one-solvent and two-solvent recrystallizations

薄层分析法在许多研究实业室是最常用的此影片将带领你每 步骤,从准备跑瓶到计算RF(retention factor)值 步骤, Thin-layer chromatography is the most commonly used analytical technique in many research labs. This video walks you through every step from setting up a developing chamber to calculating Rf values

Overview: As you may discover, growing single crystals takes patience as well as an artful hand Crystallizations can be very sensitive to temperature and minor disturbances. Therefore, you will be encouraged to try several different temperatures using otherwise identical conditions, and to always find a quiet undisturbed location to promote crystal growth. Here are some tried and true tips to get