1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)

1、营销渠道是什么(What is the nature of marketingChannels)？ 2、公司在设计、管理、评价和修正其渠道时将面临什么决策(What decisions do companies face in designing,managing, evaluating, and modifying their channels)？ 3、渠道的动态发展趋势是什么(What trends are takingplace in channel dynamics)？ 4、如何管理渠道的冲突(How can channel conflict bemanaged)？

Defining the Field-what do you think that economics is? (1) Subject Matter Based Definitions (2)UItra-pragmatic definitions( what economists do) (3)Methodology Based Definitions This much is certain a. Economics pretty much includes all topics related to human society

Uint I. Basic listening practice 1. Script M: Amy, sit down, we need to talk. Your mother and i are going to separate W: What! Are you crazy? You cant do that! What will my friends say? I just want to have a normal family Q: What is the girl saying?

Leading-in Activities: Discussion 1. What's your definition of money? 2. What attitude should we take toward money? 3.what’ your fantasy?

November 21, 2001 Ethical Egoism Here are three questions (of course there are others)we might want an ethical theory to answer for us: i)Which acts are right and which are wrong? Which acts ought we to perform(understandir the\ought\ as moral \ought\)? ii)What makes a particular action right or wrong? What is it about the action that determines its moral status? ii)How do we know what is right and wrong? Remember that according to relativism, whether an action is right or wrong

What is Pragmatics We human beings are odd compared with our nearest animal relatives. Unlike them, we can say what we want when we want. All normal humans can produce and understand any number of new words and sentences

Prewriting The nature of news What is news? Charles A. Dana, editor of the New york Sun, once defined it as, anything that interests a large part of the community and has never been brought to its attention before.\ In other words news is what people are talking about. News is new. As Evelyn Waugh described it, News is what the chap who doesn t care much about anything wants to read 1. Put another way, news is a change in the status quo. But does a change in the status quo guarantee

Questions on the text 1. Why and how do people change their attitude towards environment? 2. How did Canada protect its fish supply and what was the result? 3. What measures did Costa Rica take to protect its remaining forest? 4. What does the writer think of Brazil's environment protection?

Utilitarianism Last time we considered three questions one might ask an ethical theory to answer i)Which acts are right and which are wrong? Which acts ought we to perform(understanding the\ought as a moral ii)What makes a particular action right or wrong? What is it about the action that determines its moral status? 111) How do we know what is right and wrong? There are a variety of strategies for answering(iii). One might hold e. g. that moral truths are revealed by god and