第二节 原核生物RNA的转录 (RNA Transcription in prokaryots) 一、RNA聚合酶（全酶、核心酶） 二、启动子 三、转录起始 四、转录延伸 五、转录终止

2.1 染色体（chromosome） 2.1.1 染色体—遗传物质的主要载体 2.1.2 真核细胞染色体的组成 2.1.2.1 蛋白质 2.1.2.2 DNA 2.1.2.3 染色质和核小体 2. 1. 3 原核生物基因组 2.2 DNA的结构 2. 2. 1 DNA的一级结构 2. 2. 2 DNA的二级结构 2. 2. 2. 2 DNA二级结构中左手螺旋 2. 2. 3 DNA的高级结构

2.3.2 复制的起点、方向和速度 2.3.3 复制的几种主要方式 2.4 原核生物和真核生物DNA的复制特点 2.5.1 错配修复（mismatch repair） 2.5.2 碱基切除修复（Base-excision repair） 2.5.3 核苷酸切除修复（nucleotide-excision repair） 2.5.4 DNA的直接修复（direct repair）

12.1 Introduction 12.2 Replicons can be linear or circular 12.3 Origins can be mapped by autoradiography and electrophoresis 12.4 The bacterial genome is a single circular replicon 12.5 Each eukaryotic chromosome contains many replicons 12.6 Isolating the origins of yeast replicons 12.7 D loops maintain mitochondrial origins 12.8 The problem of linear replicons

四、真核生物转录水平上的基因表达调控 五、真核基因转录后水平上的调控

 掌握基因概念及结构特点；中心法则；基因转录调控相关序列；多顺反子，单顺反子；真核基因与原核基因的结构特点。  熟悉基因突变的意义  了解基因命名法

20.1 Introduction 20.2 Eukaryotic RNA polymerases consist of many subunits 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a universal factor 20.8 TBP binds DNA in an unusual way

1、遗传物质的本质 2、DNA的一级结构 3、DNA的二级结构 4、DNA物理结构的不均一性 5、DNA的变性、复性和杂交 6、DNA二级结构的多样性 7、DNA超螺旋和拓扑异构现象

9.1 Introduction 9.2 Transcription is catalyzed by RNA polymerase 9.3 The transcription reaction has three stages 9.4 A stalled RNA polymerase can restart 9.5 RNA polymerase consists of multiple subunits 9.6 RNA Polymerase consists of the core enzyme and sigma factor 9.7 Sigma factor is released at initiation 9.8 Sigma factor controls binding to DNA 9.9 Promoter recognition depends on consensus sequences

23.1 Introduction 23.2 Group I introns undertake self-splicing by transesterification 23.3 Group I introns form a characteristic secondary structure 23.4 Ribozymes have various catalytic activities 23.5 Some introns code for proteins that sponsor mobility 23.6 The catalytic activity of RNAase P is due to RNA 23.7 Viroids have catalytic activity 23.8 RNA editing occurs at individual bases