Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??

GLOSSARY Abundance of an mRNA is the average number of molecules per cell Abundant mRNAs consist of a small number of individual species, each present in a large

七、问答题 1.阐述原核生物的转录终止 (1)转录终止的两种主要的机制是什么? (2)描述翻译怎样能调节转录终止

三、填空题 1 由核糖核苷酸通过 键连接而成的一种

Occurs The borders between introns and exons are marked by specific nucleotide sequences within the pre-mRNAS

There are genetic processes that rearrange DNA sequences and thus lead to a more dynamic genome structure

All DNA is recombinant DNA. Genetic exchange works constantly to blend and rearrange chromosomes, most obviously during meiosis, when homologous chromosomes pair prior to the

Molecular Biology course CHAPTER 9: The mutability and repair of DNA