1.1 The Definition of Metabolism The highly integrated network of chemical transformations. Degradation (decreasing order, thus energy releasing) to provide energy, catabolism; Synthesis (increasing order, thus energy consuming) to provide building materials, anabolism

dissociation and reconstitution studies Abundant knoblike protruding structures were observed on the matrix side of the inner mitochondrial membrane by EM (Racker in 1960). The inside-out submitochondrial particles with the knobs\ are capable of both electron transferring and ATP synthesis

The cellular respiration (complete oxidation of fuels) can be divided into three stages Stage I All the fuel molecules are oxidized to generate a common two-carbon unit, acetyl-CoA. Stage II The acetyl-CoA is completely oxidized

Chapter 25 DNA Metabolism 1. How does a DNA molecule replicate with high fidelity? 2. How are DNA lesions (damages)repaired to maintain the integrity of genetic information? 3. How do DNA molecules recombine (rearrange)? High accuracy, multitude of participants

Assay:化验,分析,测定。 Enzyme activity: unit() is defined as the amount of enzyme which convert 1 micromole (umol) of substrate to product(s) in 1 minute under specified conditions. Specific activity: enzyme activity expressed per unit mass of protein present. (units/mg)

1. How does a DNA molecule replicate withhigh fidelity? 2. How are DNA lesions(damages) repairedto maintain the integrity of geneticinformation? 3. How do dna molecules recombine(rearrange)?High accuracy, multitude of participants

12.1 Synthesis and processing of bacterial RNAs 12.2 Synthesis and processing of eukaryotic RNA

Lipids are a class of nonpolar molecules that include the fats, cholesterol, fatty acids, lipid-soluble vitamins, waxes, soaps, glycerophospholipids, sphingolipids, and others. Lipids are found in the membranes of cells, the endoplasmic reticulum and in specialized fat storage cells called adipocytes. Example of lipids

Much of the catalytic power of enzymes comes from their bringing sub- strates together in favorable orientations in enzyme-substrate (ES)com- plexes. The substrates are bound to a specific region of the enzyme called the active site. Most enzymes are highly selective in their binding

Announcement \Chapter 8 Enzymes: Basic Concepts and Kinetics\ in Lubert Stryer's \Biochemistry\ (Fourth Edition) will be used as my major lecture material, which is available for you to Xerox from your TAs. Chapter 8 in Lehninger's“ Principles of Biochemistry can be used as a reference