Transcription is very similar to DNA replication but there are some important differencesi 1 RNA is made of ribonucleotides 2. RNA polymerase catalyzes the reaction 3. The synthesized RNa does not remain base-paired to the template DNA strand 4. Less accurate(error rate: 10-4)
Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed
Chapter 5A. The Movement of Substances Across cell membranes Learning Obiectives 1. Principles of membrane transport 2. Passive transport and active transport; 3. Two main classes of membrane transport proteins Carriers and Channels
Chapter 7 Energy Generation in Mitochondria and Chloroplasts (1)Mitochondria: in all eukaryotic cells The relationship between the structure and function of mit. ()Chloroplasts: in plant cells The relationship between the structure and function of chI. Mit: Oxidative phosphorylation→atp hl: Photosynthesis→atp+ NADPH→ Sugar
Chapter 1 Introduction to the cell Learning Objectives 1. About Cell Biology 2. Look briefly at the history of cell theory; 3. Consider the basic properties of cells; 4. Compare some characteristics of two different classes of cells: prokaryotes and eukaryotes; 5.comprehend a special life: viruses
Chapter 11 Cell proliferation and its regulation Significance: 1. For the growth and development of a multicellular organism, and for the generation of offspring; 2. Produce new organisms in unicellular species; 3. Renew the aging, apoptotic cells, and damaged tissue;
