第一节 转录 Transcription 第二节 转译 translation 第三节 遗传密码 The Genetic Code 第四节 遗传密码的破译 Cracking the code 第五节 真核生物RNA

Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer

一、胺的结构 氨和胺分子呈角锥型，N：sp3 杂化 苯胺：N：接近sp3 杂化，形成p-π共轭体系

Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?

10.1晶休结构和类型 10.2金属晶休 10.3离子晶休 10.4分子晶 10.5层状晶体

9.1价键理论 9.2价层电子对互斥理论 9.3分子轨道理论 9.4键参数

Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

一、刚性双原子分子 二、分子平均平动动能