1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)

Quiz: Section K, L, M and N, 40 min, English answer only 1. Please list the important cis-acting elements and transacting enzymes/factors in prokaryotic transcription including initiation, elongation and termination, and briefly describe their corresponding roles (35 point)

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)

1. RNA polymerase and transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes •Molecular Biology Course

高中物理选修人教版精品课件_第7章 分子动理论_4.温度和温标

Topic 1: THE CODE IS DEGENERATE Topic 2: THREE RULES GOVERN THE GENETIC CODE Topic 3: SUPPRESSOR MUTATIONS CAN RESIDE IN THE SAME OR A DIFFERENT GENE Topic 4: THE CODE IS NEARLY UNIVERSAL

高中物理选修人教版精品课件_7.3 分子间的相互作用力 （人教版选修3-3）

Preparation, analysis and manipulation of nucleic acids and proteins

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods