《医学分子生物学 Molecular Biology in Medicine》课程PPT教学课件：第二章 核酸和蛋白质的结构与功能 Structures &functions ofnucleic acid &protein

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

1.DNA Structure 2.DNA Topology 3.RNA Structure

Preparation, analysis and manipulation of nucleic acids and proteins

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Topic 1: THE CODE IS DEGENERATE Topic 2: THREE RULES GOVERN THE GENETIC CODE Topic 3: SUPPRESSOR MUTATIONS CAN RESIDE IN THE SAME OR A DIFFERENT GENE Topic 4: THE CODE IS NEARLY UNIVERSAL

1. RNA polymerase and transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes •Molecular Biology Course

Quiz: Section K, L, M and N, 40 min, English answer only 1. Please list the important cis-acting elements and transacting enzymes/factors in prokaryotic transcription including initiation, elongation and termination, and briefly describe their corresponding roles (35 point)

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)