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一、转录反应和转录方向 二、摸板链(-)和编码链(+)p.166 三、转录步骤:起始,延伸,终止
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How Important Is Cost? Reagents obviously figure into the cost of a procedure, but the labor required to produce and apply the reagents of purification should also be considered. How Important Is Reproducibility (Robustness) of the Procedure?
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1. Please describe the main features of the DNA structure. (10 points) Key features: (1) Double helical structure (4’) (2) Two strands are held together in an antiparallel orientation (3’) by base paring (1’)
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Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones
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Recombinant gene expression in eukaryotic systems is often the only viable route to the large-scale production of authentic, post￾translationally modified proteins. It is becoming increasingly easy to find a suitable system to overexpress virtually any gene product
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一、三种不同的RNA聚合酶 二、RNA 聚合酶的组成 三、真核RNA聚合酶的活性
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Part I: Describe the following 10 terms (3 points each). (1) EF-G: a. its structure likes tRNA b. it is a translational elongation factor c. it binds to ribosome when associated with GTP
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Q1: Please name the following amino acids (three letter name, for example: Ala for alanine). Q2: Which of them are hydrophobic AA, and which are hydrophilic?
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Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)
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1. RNA polymerase and transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes •Molecular Biology Course
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