一、基因治疗的策略 The Strategy of Gene Therapy 二、基因转移技术 The Technique of Gene Transfer 三、基因干预 Gene Interference 四、治疗基因的受控表达 Regulated Expression of Therapeutic Gene 五、基因治疗的应用研究 The Application of Gene Therapy

1. What is gene therapy? 2. How does gene therapy work? 3. The current status of gene therapy research 4. Factors kept gene therapy effective 5. Some recent developments 6. Some of the ethical considerations

1. What is gene therapy? 2. How does gene therapy work? 3. The current status of gene therapy research 4. Factors kept gene therapy effective 5. Some recent developments 6. Some of the ethical considerations

第一节 基因诊断 Gene Diagnosis 第二节 基因治疗 Gene Therapy

第一节 引言 第二节 基因注释数据库 Gene Annotation Database 第三节 基因集功能富集分析 Gene Set Enrichment Analysis 第四节 基因功能预测 Gene Function Prediction

第一节 概述 (Introduction) 第二节 原核生物基因表达的调控(Control of Prokaryotic Gene Exepression ) 第三节 真核生物基因表达的调控(Control of Eukaryotic Gene Exepression ) 第二节 真核生物基因表达的调控(Control of Eukaryotic Gene Exepression ) DNA水平的调控 转录水平的调控(transcriptional regulation) 转录后水平的调控(post transcriptional regulation) 翻译水平的调控(translational regulation) 翻译后水平的调控(protein maturation)

1. Gene Chemistry 2. Gene Structure 3. DNA replication 4. Gene Expression

第一节 基因表达与基因表达调控的基本概念与特点 Basic Conceptions and Characters of Gene Expression and it’s Regulation 第二节 原核基因表达调控 Regulation of Gene Expression in Prokaryote 第三节 真核基因表达调控 Regulation of Gene Expression in Eukaryote

Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed

第一节 基本概念与原理 Basic Conceptions and Principle 第二节 原核基因转录调节 Regulation of Prokaryotic Gene Transcription 第三节 真核基因转录调节 Regulation of Eukaryotic Gene Transcription