Preparation, analysis and manipulation of nucleic acids and proteins

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Topic 1: THE CODE IS DEGENERATE Topic 2: THREE RULES GOVERN THE GENETIC CODE Topic 3: SUPPRESSOR MUTATIONS CAN RESIDE IN THE SAME OR A DIFFERENT GENE Topic 4: THE CODE IS NEARLY UNIVERSAL

Quiz: Section K, L, M and N, 40 min, English answer only 1. Please list the important cis-acting elements and transacting enzymes/factors in prokaryotic transcription including initiation, elongation and termination, and briefly describe their corresponding roles (35 point)

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)

1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)

Q1: Please name the following amino acids (three letter name, for example: Ala for alanine). Q2: Which of them are hydrophobic AA, and which are hydrophilic?

1. Please describe the main features of the DNA structure. (10 points) Key features: (1) Double helical structure (4’) (2) Two strands are held together in an antiparallel orientation (3’) by base paring (1’)

Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones

PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary