1. Recognizing the life of scientists. 2. Recognizing the value and nature of science. 3. Recognizing the characters displayed during the scientific activity: love of the beauty of nature, the faith of your creativity and the hope to be recognized

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Preparation, analysis and manipulation of nucleic acids and proteins

PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary

How Important Is Cost? Reagents obviously figure into the cost of a procedure, but the labor required to produce and apply the reagents of purification should also be considered. How Important Is Reproducibility (Robustness) of the Procedure?

Recombinant gene expression in eukaryotic systems is often the only viable route to the large-scale production of authentic, post￾translationally modified proteins. It is becoming increasingly easy to find a suitable system to overexpress virtually any gene product

一、三种不同的RNA聚合酶 二、RNA 聚合酶的组成 三、真核RNA聚合酶的活性

Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones

一、转录反应和转录方向 二、摸板链（-）和编码链（+）p.166 三、转录步骤：起始,延伸,终止