Restriction enzymes are used for cutting DNA at precise locations Vectors are used for the propagation of cloned DNA fragments Libraries of cloned DNA fragments allow searching for a specific DNA fragment

基因类别 结构基因包括编码结构蛋白和酶蛋白的基因, 也包括编码阻遏蛋白和激活蛋白的基因。 调控基因包括调节基因、启动基因和操纵基因

How DNA as genetic materials was established (also summarized in er including structure and components Basic properties of DNA, i xt slide)

There are many ways of cloning a disease gene, depending on what is known about the disease. We will show several typical examples in this part of the lecture

Bacterial Genetics Key Requirements Understand how genetic mapping is achieved in bacteria: Co-transformation, F factor Hfr strain co-transduction and some understanding of phage genetics 2. Read the textbook and do the problem set at the back

第一节基因突变的时期和特征 一、基因突变的时期 二、基因突变的一般特征

1 分离规律、自由组合规律的定义,遗传现象, 表现形式和实质 2 实现孟德尔分离比的条件 3 用分枝法对多基因杂种进行遗传分析

1 题解: 名词解释 P163 2 题解: 据题意 10/200=5% 3 题解: 据交换值愈大，两个基因在染色体上相距愈

Using mutants to analyze the pathway or functions of genes Mutants have to be generated to analyze the pathway or functions of genes Saturation screening Overexpression Suppressor mutations Synthetic lethals Nonallelic noncomplementation

一、概念：重复；缺失；倒位；易位；假显性；假连锁；交换抑制因子；Schultz-Redfield效应；平衡致死系；染色体组；单倍体；二倍体；多倍体；单体；缺体；三体；四体；双三体