H protein synthesis H1 The genetic code H2 Protein synthesis(translation) in prokaryotes H3 Translation in eukaryotes H4 Protein targeting H5 Protein glycosylation

1. A brief history of understanding protein metabolism; 2. The studies leading to the deciphering of the genetic codes; 3. The pathway leading to the synthesis of a functional protein; 4. Current understanding on protein targeting and degradation

第一节 蛋白质生物合成体系 Protein Biosynthesis System 第二节 氨基酸与tRNA的连接 第三节 肽链的生物合成过程 第四节 蛋白质翻译后修饰和靶向输送 Posttranslational Modification and Targeting Transfer of Protein 第五节 蛋白质生物合成的干扰和抑制 Interference and Inhibition of Protein Biosynthesis

1. Hemoglobin is a multisubunit allosteric protein that carries 02 in erythrocyte. 1.1 Hemoglobin is a well-studied and well-understood protein. 1.1.1 It was one of the first proteins to have its molecular mass accurately determined. 1.1.2 The first protein to be characterized by ultracentrifuge. 1.1.3 The first protein to be associated with a specific physiological function

1. System of protein biosynthesis 2. Process of protein translation 3. Post-translational modification 4. Inhibitors of protein synthesis

5.1 Reversible Binding of a Protein to a Ligan proteins interact with other molecules and how their in- Oxygen-Binding Proteins 158 orotein structure. The 5.2 Complement protein's func- and Ligands: The Immune Immunoglobulins 174 5.3 Protein Interactions Modulated by Actin, Myosin, and

• 分子生物学和医学分子生物学研 究的主要内容 • 分子生物学的历史 • 分子生物学在医学上的应用 • Nucleic acids DNA Structures &functions RNA Structures &functions • Proteins • Interaction between biopolymer protein-protein interaction DNA-protein interaction

Outline 6.1 Forces Influencing protein structure 6.2 Role of the amino acid sequence in Protein structure 6.3 Secondary structure of proteins 6.4 Protein Folding and tertiary structure 6.5 Subunit Interactions and Quaternary Structure

NMR supplement Kinetic studies of protein folding using NMR spectroscopy Christopher M. Dobson and Peter J Hore Recent progress has advanced our abilities to use NMR spectroscopy to follow- in real time -the structural and dynamic changes taking place during protein folding

1. Overview-the many levels of systems biology 2. Experimental methods for measuring protein-protein interactions and their limitations 3. Data sources for information about proteins and their