第十章细菌及病毒的遗传分析(2h) 第一节细菌和病毒遗传研究的意义 第二节噬菌体的基因重组 第三节细菌基因重组

遗传学( Genetics)是研究生物遗传和变异的科学 生物有许多特征区别于非生物,但是最重要的是以下两点:第一是生存,第二 是繁衍种族 种瓜得瓜,种豆得豆。这种世代间相似的现象就是遗传 heredity)。 母生九子、各各不一样。同一物种内个体间的差异在遗传学上称为变异 ( Variation)

About the final It will be in English. Try to answer in English as well. The problems are given in such a way that very short answers are needed. Chinese allowed if you can only read English

F and partial diploids The F-factor in a Hfr chromosome can excise out to become an autonomously replicating plasmid If a segment of the chromosome becomes part of the F pp lasmid during excision, the new element is called an F plasmid

Bacterial Genetics Key Requirements Understand how genetic mapping is achieved in bacteria: Co-transformation, F factor Hfr strain co-transduction and some understanding of phage genetics 2. Read the textbook and do the problem set at the back

第一节遗传工程概述 遗传工程广义:细胞工程、染色体工程 细胞器工程、基因工程

Restriction enzymes are used for cutting DNA at precise locations Vectors are used for the propagation of cloned DNA fragments Libraries of cloned DNA fragments allow searching for a specific DNA fragment

How DNA as genetic materials was established (also summarized in er including structure and components Basic properties of DNA, i xt slide)

基因类别 结构基因包括编码结构蛋白和酶蛋白的基因, 也包括编码阻遏蛋白和激活蛋白的基因。 调控基因包括调节基因、启动基因和操纵基因

There are many ways of cloning a disease gene, depending on what is known about the disease. We will show several typical examples in this part of the lecture