武汉大学生命科学学院：《分子生物学》（英文版）Intro 课程特点与目标 Mission & Goal

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

1.DNA Structure 2.DNA Topology 3.RNA Structure

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

TOPIC 1 Principles of Transcriptional Regulation [watch the animation] TOPIC 2 Regulation of Transcription Initiation: Examples from Bacteria (Lac operon, alternative s factors, NtrC,MerR, Gal rep, araBAD operon) TOPIC 3 Examples of Gene Regulation after Transcription Initiation (Trp operon) TOPIC 4 The Case of Phage λ: Layers of Regulation

◼ What are the main challenges of translation and how do organisms overcome them? ◼ What is the organization of nucleotide sequence information in mRNA? ◼ What is the structure of tRNAs, and how do aminoacyl tRNA synthetases recognize and attach the correct amino acids to each tRNA? ◼ How does the ribosome orchestrate the translation process?

1. RNA polymerase and transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes •Molecular Biology Course

◼ All DNA is recombinant DNA. ◼ Genetic exchange works constantly to blend and rearrange chromosomes, most obviously during meiosis, when homologous chromosomes pair prior to the first nuclear division. ◼ During this pairing, genetic exchange between the chromosomes occurs. This exchange, classically termed crossing over, is one of the results of homologous recombination

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)

1. Please define the term “mutation”, and list the major types of DNA mutations and their corresponding phenotypic effects. (20 points) 2. Please define the term “DNA lesion”. Please list the three types of DNA lesions and the chemical or physical mutagens cause these lesions. (20 points)