9.1 Introduction 9.2 Transcription is catalyzed by RNA polymerase 9.3 The transcription reaction has three stages 9.4 A stalled RNA polymerase can restart 9.5 RNA polymerase consists of multiple subunits 9.6 RNA Polymerase consists of the core enzyme and sigma factor 9.7 Sigma factor is released at initiation 9.8 Sigma factor controls binding to DNA 9.9 Promoter recognition depends on consensus sequences

20.1 Introduction 20.2 Eukaryotic RNA polymerases consist of many subunits 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a universal factor 20.8 TBP binds DNA in an unusual way

Comparison of Meningococcus and Gonococcus Pathobiology Similarities: – Both diploccocci – Share about 80% DNA – Neither makes exotoxin; both make endotoxin – Both are relatively good colonizers

ADNA的体外重组(切、接) B重组DNA分子的转化和扩增(转、增) C转化子的筛选和鉴定(检)

第一节 痘病毒科 第二节 非洲猪瘟病毒科 第三节 疱疹病毒科 第四节 腺病毒科

第一节 痘病毒科 第二节 非洲猪瘟病毒科 第三节 疱疹病毒科 第四节 腺病毒科

核酸中的碱基(Base in Nucleic Acids) DNA——Deoxyribonucleic acid

A重组DNA技术的理论基础 B基因的分子生物学 C基因工程的基本原理 D基因工程的支撑技术

遗传的细胞学基础 细胞的结构 主要的细胞器及功能 染色体/染色质 细胞分裂 遗传的物质基础 核酸是遗传物质 DNA RNA

一、染色体畸变 二、基因突变 三、突变的抑制与DNA的修复 四、重组与转座 遗传信息的改变