17.1 Introduction 17.2 The mating pathway is triggered by pheromone-receptor interactions 17.3 The mating response activates a G protein 17.4 Yeast can switch silent and active loci for mating type 17.5 The MAT locus codes for regulator proteins 17.6 Silent cassettes at HML and HMR are repressed 17.7 Unidirectional transposition is initiated by the recipient MAT locus 17.8 Regulation of HO expression 17.9 Trypanosomes switch the VSG frequently during infection 17.10 New VSG sequences are generated by gene switching 17.11 VSG genes have an unusual structure 17.12 The bacterial Ti plasmid causes crown gall disease in plants 17.13 T-DNA carries genes required for infection 17.14 Transfer of T-DNA resembles bacterial conjugation 17.15 Selection of amplified genomic sequences 17.16 Transfection introduces exogenous DNA into cells 17.17 Genes can be injected into animal eggs 17.18 ES cells can be incorporated into embryonic mice 17.19 Gene targeting allows genes to be replaced or knocked out

16.1 Introduction 16.2 The retrovirus life cycle involves transposition-like events 16.3 Retroviral genes codes for polyproteins 16.4 Viral DNA is generated by reverse transcription 16.5 Viral DNA integrates into the chromosome 16.6 Retroviruses may transduce cellular sequences 16.7 Yeast Ty elements resemble retroviruses 16.8 Many transposable elements reside in D. melanogaster 16.9 Retroposons fall into two classes 16.10 The Alu family has many widely dispersed members

12.1 Introduction 12.2 Replicons can be linear or circular 12.3 Origins can be mapped by autoradiography and electrophoresis 12.4 The bacterial genome is a single circular replicon 12.5 Each eukaryotic chromosome contains many replicons 12.6 Isolating the origins of yeast replicons 12.7 D loops maintain mitochondrial origins 12.8 The problem of linear replicons

Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed

四、真核生物转录水平上的基因表达调控 五、真核基因转录后水平上的调控

一、基因与基因表达的一般概念 二、遗传密码三联子 三、密码子和反密码子的相互作用 四、tRNA 五、AA-tRNA合成酶 六、核糖体 七、信使核糖核酸 八、蛋白质的生物合成 九、氨基酸及功能蛋白质合成后的修饰 土、蛋白质的运输和降解

浙江省浙南、浙北部分学校2012届高三第一学期12月联考语文试题_浙江省浙南、浙北部分学校2012届高三12月联考（语文）

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