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Outline 4.1: Amino Acids: Building Blocks of Proteins 4.2: Acid-Base Chemistry of Amino Acids 4.3: Reactions of Amino Acids 4.4: Optical Activity and Stereochemistry of Amino Acids 4.5: Spectroscopic Properties of Amino Acids 4.6: Separation and Analysis of a.a. Mixtures

Outline 6.1 Forces Influencing protein structure 6.2 Role of the amino acid sequence in Protein structure 6.3 Secondary structure of proteins 6.4 Protein Folding and tertiary structure 6.5 Subunit Interactions and Quaternary Structure

Primary Structure Sequencing Nucleic Acids核酸序列测定 Chain termination method (dideoxy method), developed by F. Sanger Base-specific chemical cleavage, developed by Maxam and Gilbert Both use autoradiography- X-ray film develops in response to presence of radioactive isotopes in nucleic acid molecules