Using mutants to analyze the pathway or functions of genes Mutants have to be generated to analyze the pathway or functions of genes Saturation screening Overexpression Suppressor mutations Synthetic lethals Nonallelic noncomplementation
There are many ways of cloning a disease gene, depending on what is known about the disease. We will show several typical examples in this part of the lecture
F and partial diploids The F-factor in a Hfr chromosome can excise out to become an autonomously replicating plasmid If a segment of the chromosome becomes part of the F pp lasmid during excision, the new element is called an F plasmid
Bacterial Genetics Key Requirements Understand how genetic mapping is achieved in bacteria: Co-transformation, F factor Hfr strain co-transduction and some understanding of phage genetics 2. Read the textbook and do the problem set at the back
