How Important Is Cost? Reagents obviously figure into the cost of a procedure, but the labor required to produce and apply the reagents of purification should also be considered. How Important Is Reproducibility (Robustness) of the Procedure?

Recombinant gene expression in eukaryotic systems is often the only viable route to the large-scale production of authentic, post￾translationally modified proteins. It is becoming increasingly easy to find a suitable system to overexpress virtually any gene product

DNA转录RNA前体加工成熟RNA的过程 RNA合成的两种方式: 1、DNA指导的RNA合成。 2、RNA指导的RNA合成

通过各种方法使酶分子的结构发生某些改变,从而改变酶的某些特性和功能的过程称为酶分子修饰酶分子是具有完整的化学结构和空间结构的生物大分子。酶分子的结构决定了酶的性质和功能。 正是酶分子的完整空间结构赋予酶分子以生物催化功能,使酶具有催化效率高、专一性强和作用 条件温和等特点。但是在另一方面,也是酶的分 子结构使酶具有稳定性较差、活性不够高和可能 具有抗原性等弱点 当酶分子的结构发生改变时将会引起酶的性质和功能的改变

烯烃：分子中含碳碳双键的烃。通式为CnH2n。 碳碳双键是烯烃的官能团

Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary. 1. Yeast artificial chromosome 2. RNA interference 3. Proteomics

PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary

一.反应的类型 二.反应机理 1.E1机理 2.单分子共轭碱消除(E1CB机理 CB) 3.E2机理

I Classification of Rearrangement Reactions() II Nucleophilic rearrangement() 1 Wagner-Meerwein rearrangement( 2 Pinacolic Rearrangement 3 a-ethandione Rearrangement 4 Beckmann Rearrangement 5 Baeyer-Villiger Rearrangement

Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones