本章主要的学习的内容为三节内容：第一节：核酸的结构；1、核苷酸；2、核酸的共价结构；3、DNA的高级结构；4、RNA的高级结构；第二节：核酸的物化性质；1、核酸的水解；2、核酸的酸碱性质；3、核酸的紫外吸收性质；4、核酸的变性、复性及分子杂交

1、主要在肝脏中进行的代谢途径：糖异生、胆汁酸 生成、酮体生成、胆固醇合成、尿素生成某些血浆蛋 白(清蛋白、凝血酶原、纤维蛋白原、α1-抗胰蛋白酶等 )合成 、核苷酸的从头合成、生物转化、激素灭活、维 生素加工

一、钙、磷在体内的分布与存在形式 1、钙、磷的主要存在形式:磷酸钙和羟磷灰石 2、血钙与血磷的关系:[Ca][P]=35~40

又称遗传操作。广义的包括细胞工程、 染色体工程和基因工程，狭义的仅指基 因工程。是以遗传学特别是分子遗传学 为理论基础，以现代物理学、化学等技 术手段，按照人们设计的生物蓝图，在 细胞、染色体和基因的不同水平上，对 生物的遗传性进行定向的改造。遗传工 程在人类健康和工业、农业、环境保护 等方面有着广阔的应用前景

Over the past decade the variety of hosts and vector systems for recombinant protein expression has increased dramatically. Researchers now select from among mammalian, insect, yeast, and prokaryotic hosts, and the number of vectors available for use in these organisms continues to grow

What Are the Symptoms of Acrylamide Poisoning? The initial symptoms of acrylamide poisoning on the skin are peeling of the skin at the point of contact, followed by tingling and numbness in the exposed area. If exposure by any means (touch, ingestion, inhalation) continues, muscular weakness, difficulty maintaining balance

Companies hire researchers, license ideas, generate much useful data that aren’t always published, and fund scholarships. Famil￾iarity with the corporate mindset, structure and resources can help you obtain what you need and avoid problems you don’t want. All Companies Are the Same?

Air Buoyancy Akin to water keeping something afloat, samples can be “lifted” by air, artificially decreasing their apparent weight. This air buoy￾ancy can have a significant effect on smaller samples. Electrostatic Forces Electrostatic charges are almost always present in any environ￾ment, particularly in areas with very low humidity. If there are

What Do You Know About Your Target? The sensitivity needs of your system are primarily determined by the abundance of your target, which can be approximated according to its origin. Plasmids, cosmids, phagemids as colony lifts or dot blots, and PCR products are usually intermediate to high-abundance targets. Genomic DNA is considered an intermediate to low-abundance target. Most prokaryotic genes are present as single copies, while genes from higher eukaryotes

The principle of the polymerase chain reaction (PCR) was first reported in 1971 (Kleppe et al., 1971), but it was only after the dis￾covery of the thermostable Taq DNA polymerase (Saiki et al., 1988; Lawyer et al., 1989) that this technology became easy to use