第一节细胞信息传递概述 第二节受体 第三节细胞信息的传递途径及其分子机理 第四节信息传递途径的交互联系

How Important Is Cost? Reagents obviously figure into the cost of a procedure, but the labor required to produce and apply the reagents of purification should also be considered. How Important Is Reproducibility (Robustness) of the Procedure?

Recombinant gene expression in eukaryotic systems is often the only viable route to the large-scale production of authentic, post￾translationally modified proteins. It is becoming increasingly easy to find a suitable system to overexpress virtually any gene product

Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary. 1. Yeast artificial chromosome 2. RNA interference 3. Proteomics

Q1: Please name the following amino acids (three letter name, for example: Ala for alanine). Q2: Which of them are hydrophobic AA, and which are hydrophilic?

Quiz: Section K, L, M and N, 40 min, English answer only 1. Please list the important cis-acting elements and transacting enzymes/factors in prokaryotic transcription including initiation, elongation and termination, and briefly describe their corresponding roles (35 point)

Topic 1: THE CODE IS DEGENERATE Topic 2: THREE RULES GOVERN THE GENETIC CODE Topic 3: SUPPRESSOR MUTATIONS CAN RESIDE IN THE SAME OR A DIFFERENT GENE Topic 4: THE CODE IS NEARLY UNIVERSAL

1. RNA polymerase and transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes •Molecular Biology Course

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)

1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)