F and partial diploids The F-factor in a Hfr chromosome can excise out to become an autonomously replicating plasmid If a segment of the chromosome becomes part of the F pp lasmid during excision, the new element is called an F plasmid
There are many ways of cloning a disease gene, depending on what is known about the disease. We will show several typical examples in this part of the lecture
Restriction enzymes are used for cutting DNA at precise locations Vectors are used for the propagation of cloned DNA fragments Libraries of cloned DNA fragments allow searching for a specific DNA fragment