1. Please describe the main features of the DNA structure. (10 points) Key features: (1) Double helical structure (4’) (2) Two strands are held together in an antiparallel orientation (3’) by base paring (1’)

Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones

PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary

《医学分子生物学 Molecular Biology in Medicine》课程PPT教学课件：第十七章 基因治疗

Translation is the conversion of the information in the nucleic acid sequence to polypeptides of a specific amino acid sequence

一、三种不同的RNA聚合酶 二、RNA 聚合酶的组成 三、真核RNA聚合酶的活性

一、转录反应和转录方向 二、摸板链（-）和编码链（+）p.166 三、转录步骤：起始,延伸,终止

以DNA或RNA为诊断材 料，通过检查基因的存在、缺陷或 表达异常，对人体状态和疾病作出 诊断的方法和过程

聚合酶链式反应 连接酶链反应 RNA的体外扩增

•Virus genome •Prokaryote Genome •Eukaryote Genome