13.1 Introduction 13.2 DNA polymerases are the enzymes that make DNA 13.3 DNA synthesis is semidiscontinuous 13.4 Coordinating synthesis of the lagging and leading strands 13.5 The replication apparatus of phage T4 13.6 Creating the replication forks at an origin 13.7 Common events in priming replication at the origin 13.8 Does methylation at the origin regulate initiation? 13.9 Licensing factor controls eukaryotic rereplication

第一节 概论 第二节 DNA的分子结构 第三节 DNA的二级结构 第四节 DNA的三、高级结构 第五节 RNA的结构与功能 第六节 核酸的理化性质

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

真核细胞的染色体由()、()和()组成。 DNA复制时需要()将DNA双链解开,靠()维持单链结构

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

第一节 DNA作为遗传物质的证据 第二节 核酸的化学结构和复制 第三节 DNA与遗传密码 第四节 DNA与蛋白质合成

一、历史回顾 140年前, Mendel在捷克莫勒温镇一个修道院里沉醉于豌豆杂交实验,也许根本就没有想到,他提出的遗传因子在半个世纪后被 Morgan定义为基因。1944 Avery年证明了基因的物质基础是DNA50年代 WatsonCrick初和又揭示了DNA的分子结构70年代初,DNA已可在体外被随意拼接并转回到细菌体内遗传和表达

Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed

Single-Stranded Filamentous DNA Bacteriophages M13, fl and fd Distinct Properties of M13 -Infect Escherichia coli Infect only male cells through attachment to the male-specific pilus Have circular single-stranded DNA Can be used as a cloning vector and DNA sequencing vehicle in genetic engineering Infect but not kill the cells

专题七DNA与基因表达调控 7.1DN生命的秘密与基因 7.2基因表达与调控