Primary Structure Sequencing Nucleic Acids核酸序列测定 Chain termination method (dideoxy method), developed by F. Sanger Base-specific chemical cleavage, developed by Maxam and Gilbert Both use autoradiography- X-ray film develops in response to presence of radioactive isotopes in nucleic acid molecules
Outline 11.1 Nitrogenous Bases 11. The pentoses of nucleotides and na 11.3 Nucleosides are Formed by joining a Nitrogenous Base to a Sugar 11.4 Nucleotides -Nucleoside Phosphates 11.5 Nucleic Acids are Polynucleotides 11.6 Classes of nucleic acids 11.7 Hydrolysis of Nucleic acids
Outline 5.1 Proteins Linear Polymers of Amino acids 5.2 Architecture 5.3 Many Biological Functions 5.4 May be Conjugated with Other Groups 5.6 Purification 5.7 Primary Structure Determination 5.8 Consider the Nature of Sequences
Outline 4.1: Amino Acids: Building Blocks of Proteins 4.2: Acid-Base Chemistry of Amino Acids 4.3: Reactions of Amino Acids 4.4: Optical Activity and Stereochemistry of Amino Acids 4.5: Spectroscopic Properties of Amino Acids 4.6: Separation and Analysis of a.a. Mixtures