(一)基因工程的概念 1.遗传工程广义;细胞工程-体细胞、原生质体的培养与杂交细器---细胞核的移植:回交,克隆羊染色体----条染色体或染色体片断的切割或转移基因工程狭义:基因工程:体外不经过有性繁殖,有目的地使DNA发生重组的技术体系。故又称重组DNA技术

once oncu n a paiuicuia positon on DNA, a nucleosome remained fixed in place because of the tight association 30-nm 98A5 sequence-specific DNA-binding proteins nucleosome

A重组DNA技术的理论基础 B基因的分子生物学 C基因工程的基本原理 D基因工程的支撑技术

ADNA的体外重组(切、接) B重组DNA分子的转化和扩增(转、增) C转化子的筛选和鉴定(检)

1.复制的特征 2.DNA复制的酶学 3.复制的过程 4.DNA损伤与修复 5.逆转录作用

Chapter 5 Macromolecules and Molecular Genetics The key informational macromolecules in prokaryotic and eukaryotic cells are DNA, RNA, and protein. Molecular Genetics deals with the mechanisms by which the information in DNA is replicated, and by which it can be transcribed into rNa and translated into specific proteins

一、物理性质 1、形态DNA为白色纤维状固体;RNA为白色粉末状固体? 2、溶解性微溶于水,不溶于乙醇、乙醚和氯仿等一般的有机溶剂;DNA在溶液中粘度大,RNA粘度小

Chapter 5 Macromolecules and Molecular Genetics DNA Replication Complementary base pairing Semiconservative Template and primer From 5'-phosphate to the 3'-hydroxyl end DNA polymerase and ligase A short stretch of RNA serves as primer Primase: a specific RNA-polymerizing enzyme

Arrangement of DNA in Prokaryotes Vertebrates Algae Fungi Free-living prokaryotes Obligately parasitic Bacteria Chloroplasts Nudleod Mitochondria Viruses

第一节重组DNA向宿主细胞的导入 第二节转化子的筛选与鉴定 第三节目的基因序列测定