概述 肝细胞结构与功能的异质性(heterogeneity) 原因 不同部位的肝细胞获得的氧和营养物 质具有差异。 以终末微血管为中轴,将肝小叶中的肝细 胞分为三条带: I带(门管周带periportal zone) Ⅲ带(小叶中心带centrolobular zone) Ⅱ带(介于I带与Ⅲ带之间)

Primary Structure Sequencing Nucleic Acids核酸序列测定 Chain termination method (dideoxy method), developed by F. Sanger Base-specific chemical cleavage, developed by Maxam and Gilbert Both use autoradiography- X-ray film develops in response to presence of radioactive isotopes in nucleic acid molecules

Outline 11.1 Nitrogenous Bases 11. The pentoses of nucleotides and na 11.3 Nucleosides are Formed by joining a Nitrogenous Base to a Sugar 11.4 Nucleotides -Nucleoside Phosphates 11.5 Nucleic Acids are Polynucleotides 11.6 Classes of nucleic acids 11.7 Hydrolysis of Nucleic acids

Outline 5.1 Proteins Linear Polymers of Amino acids 5.2 Architecture 5.3 Many Biological Functions 5.4 May be Conjugated with Other Groups 5.6 Purification 5.7 Primary Structure Determination 5.8 Consider the Nature of Sequences

Outline 4.1: Amino Acids: Building Blocks of Proteins 4.2: Acid-Base Chemistry of Amino Acids 4.3: Reactions of Amino Acids 4.4: Optical Activity and Stereochemistry of Amino Acids 4.5: Spectroscopic Properties of Amino Acids 4.6: Separation and Analysis of a.a. Mixtures