东北林业大学：高分子材料与工程专业2013级《生物质材料》课程教学大纲

In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??

Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers

Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer

Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

Chapter 3 Techniques in Cell Biology Preparatory observe put forward theoretics Design control tests Refer to knowledge Collect data Explain results Devise conclusion

Chapter 6 Cytoplasmic matrix, Endomembrane system, Protein Sorting and membrane trafficking Learning objective 1. Compartmentalization in Eukaryotic Cells 2. The structural and functional relationship among the er, Golgi complexes, lysosomes and plasma membranes ofeukaryotic cells

清华大学：《分子生物学》课程PPT教学课件（基因ene）第二十九章 梯度、级联和发信号的途径（Gradients, cascades, and signaling pathways）